Table 5 Extent of two glycosylation forms of hydroxylysine in type I collagen isolated from MC and KO clone.

From: Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes

  

Site occupancy (%)

MC

EV

KO-1

KO-2

KO-3

α1(I) K87

G-Hyl

8.2 ± 0.2

8.2 ± 0.2

12.8 ± 0.4***,###

12.6 ± 0.5***,###

8.0 ± 0.9

GG-Hyl

91.8 ± 0.2

91.8 ± 0.2

87.2 ± 0.4***,###

87.4 ± 0.5***,###

92.0 ± 0.9

α1(I) K99

G-Hyl

64.9 ± 1.0

65.5 ± 0.5

73.2 ± 0.6***,###

72.3 ± 1.1***,###

63.9 ± 0.6

GG-Hyl

35.1 ± 1.0

34.5 ± 0.8

26.8 ± 0.6***,###

27.7 ± 1.1***,###

36.1 ± 0.6

α1(I) K174

G-Hyl

56.8 ± 1.5

56.8 ± 2.2

73.0 ± 1.1***,###

71.5 ± 2.3***,###

65.8 ± 0.7***,###

GG-Hyl

43.2 ± 1.5

43.2 ± 2.2

27.0 ± 1.1***,###

28.5 ± 2.3***,###

34.2 ± 0.7***,###

α1(I) K564

G-Hyl

47.1 ± 1.4

48.9 ± 2.6

63.0 ± 1.8***,###

64.0 ± 3.5***,###

58.2 ± 1.2***,##

GG-Hyl

52.9 ± 1.4

51.1 ± 2.6

37.0 ± 1.8***,###

36.0 ± 3.5***,###

41.8 ± 1.2***,##

α2(I) K174

G-Hyl

65.3 ± 1.7

65.7 ± 0.5

76.5 ± 0.6***,###

75.1 ± 0.8***,###

63.8 ± 0.8

GG-Hyl

34.7 ± 1.7

34.3 ± 0.5

23.5 ± 0.6***,###

24.9 ± 0.8***,###

36.2 ± 0.8

α2(I) K219

G-Hyl

14.8 ± 4.5

18.6 ± 0.5

27.4 ± 5.5*

23.5 ± 4.7

16.5 ± 1.8

GG-Hyl

85.2 ± 4.5

81.4 ± 0.5

72.6 ± 5.5*

76.5 ± 4.7

83.5 ± 1.8

  1. Glycosylation of Hyl residues (%) represents the relative levels of G-Hyl, and GG-Hyl (G-Hyl + GG-Hyl = 100%).
  2. Values represent mean ± S.D. (n = 3) of triplicate analysis for each group. Statistical differences were determined by Kruskal–Wallis one-way analysis of variance and means comparison with the controls by Dunnett’s method.
  3. Hyl hydroxylysine, G- galactosyl-, GG- glucosylgalactosyl-, MC MC3T3-E1, EV empty vector, KO knock-out.
  4. *p < 0.01 and ***p < 0.001 between MC and KO; ##p < 0.01 and ###p < 0.001 between EV and KO, respectively.
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