Table 7 Levels of immature reducible cross-links (DHLNL and HLNL) and mature non-reducible cross-links (Pry and HHMD) from MC, EV, and KO clones.

From: Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes

Cells/clones

DHLNL

HLNL

Pyr

HHMD

Total aldehydes

MC

0.74 (0.01)

0.27 (0.02)

0.021 (0.004)

0.08 (0.02)

1.21 (0.05)

EV

0.61 (0.05)

0.29 (0.01)

0.018 (0.001)

0.07 (0.01)

1.07 (0.02)

KO-1

ND

0.37 (0.01)***,##

ND

0.30 (0.02)***,###

0.98 (0.02)***

KO-2

ND

0.39 (0.01) ***,###

ND

0.29 (0.01)***,###

0.97 (0.03)***,#

KO-3

ND

0.36 (0.02)***,##

ND

0.28 (0.01)***,###

0.93 (0.02)***,##

  1. Total aldehydes = DHLNL + HLNL + 2 × Pyr + 2 × HHMD. Values represent mean moles/mole collagen ± S.D. (n = 3) of triplicate analysis of the hydrolysates. Statistical differences were determined by Kruskal–Wallis one-way analysis of variance and means comparison with the controls by Dunnett’s method.
  2. DHLNL dihydroxylysinonorleucine, HLNL hydroxylysinonorleucine, HHMD histidinohydroxymerodesmosine, Pyr pyridinoline, MC MC3T3-E1, EV empty vector, KO knock-out.
  3. ***p < 0.001 between MC and KO; #p < 0.05, ##p < 0.01, and ###p < 0.001 between EV and KO, respectively.
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