Figure 3

Abundance of bile acid-relevant proteins in differentiated HuH-7 cells with overlay measured by quantitative targeted absolute proteomics (QTAP) analysis. Membrane protein abundance (pmol/mg protein; mean ± standard deviation, n = 3) of select bile acid-relevant proteins in 2-week cultured HuH-7 cells treated with 1 µM dexamethasone (DEX) and/or 0.5% DMSO was plotted as scatter plots for individual proteins. Statistically significant differences with respect to control (Ctrl) were calculated for each protein using an ordinary one-way ANOVA and Dunnett’s multiple comparison test (*, p < 0.05). N.D., not detected; LLOQ, lower limit of quantitation (0.1 pmol/mg protein).