Figure 1

Barrier function of HUCLs monitored by real-time bio impedance analysis using an AC frequency scan. HUCLs were seeded on a 96W1E + ECIS array. Three-dimensional representations of the log of normalized impedance (y-axis) as a function of both log frequency of the alternating-current (AC) (y-axis) and time (z-axis). Cells grown in DMEM/F12 and K-SFM are shown for 30,000 (A,D), 60,000 (B,E) and 100,000 (C,F) cell seeding densities. Arrows indicate start of plateau and approximate time to confluency.