Figure 6

Extremely low frequency electromagnetic field induced apoptosis. ELF-EMF induced apoptosis was examined under different frequencies (0.01 Hz, 0.1 Hz, 1 Hz, 10 Hz) and different intensities (1 mT, 10 mT, 100 mT) and different times (24 h, 48 h, 72 h, 120 h) on HUVEC, DU145, K562 and MDA-MB-231 cell lines. Histograms showing the percentage of apoptotic cells in non-exposed and exposed cells in (a) cancerous cells (DU145, MDA-MB-231) with different frequencies (0.01 Hz, 0.1 Hz, 1 Hz, 10 Hz) and intensity 100 mT for 2 h time, (b) cancerous cells (DU145, MDA-MB-231) 1 mT and 10 mT for 2 h time. (c) non-cancerous cells (HUVEC) with different frequencies (0.01 Hz, 0.1 Hz, 1 Hz, 10 Hz) and intensity 100 mT for 2 h time, (d) Non-cancerous cells (HUVEC) with frequency 0.01 Hz and intensity 1 mT and 10 mT for 2 h, (e) suspended cells with different frequencies (0.01 Hz, 0.1 Hz, 1 Hz, 10 Hz) and intensity 100 mT for 2 h time, (f) suspended cells (K562) with frequency 0.01 Hz, intensity 1 mT and 10 mT for 2 h. Different times (24 h, 48 h, 72 h, 120 h) of exposure in intensity 100 mT for (g) cancerous cells (DU145 (0.01 Hz), MDA-MB-231 (1 Hz)), (h) non-cancerous cells (HUVEC) frequency 0.01 Hz, (i) suspended cells (K562) frequency 0.01 Hz. Bars indicate (Means ± SEM) obtained from three or more independent experiments. Statistical significance between non-exposed and exposed groups was evaluated by a t-test. (P-value < 0.05) was considered to indicate statistical significance.