Table 1 Primers pairs for Gapdh, Agtr1a, Oxtr, Htr2a/2c,Tph2, Slc6a4 and Trpv1.
From: Molecular neurobiological markers in the onset of sodium appetite
Gene | GenBank access number | Forward Primer 5ʹ-3ʹ | Reverse Primer 5ʹ-3ʹ | Product lenght (bp) | Annealing temp. (°C) |
|---|---|---|---|---|---|
Gapdh | NM_017008.4 | TGTGAACGGATTTGGCCGTA | ATGAAGGGGTCGTTGATGGC | 93 | 60 |
Agtr1a | NM_030985.4 | AACCCTCTGTTCTACGGC | ACCTGTCACTCCACCTCA | 194 | 56.5 |
Oxtr | NM_012871.3 | CGTACTGGCCTTCATCGTGT | GAAGGCAGAAGCTTCCTTGG | 94 | 59.5 |
Htr2c (5Ht2C) | NM_012765.3 | TTGGACTGAGGGACGAAAGC | GGATGAAGAATGCCACGAAGG | 102 | 59.6 |
Htr2a (5Ht2A) | NM_017254.1 | AACGGTCCATCCACAGAG | AACAGGAAGAACACGATGC | 109 | 56 |
Tph2 | NM_173839.2 | CACCTGACAACATTTGGACG | ACGTTGTCCTTGAATCCTGG | 148 | 57.6 |
Slc6a4 (SerT) | NM_013034.4 | GAACTCCTGGAACACTGGCA | CAGGACATGGCGCAAGTAGA | 109 | 60 |
Trpv1 | NM_031982.1 | TTCACCGAATGGGCCTATGG | TGACGGTTAGGGGTCTCACT | 125 | 59.9 |
