Figure 3 | Scientific Reports

Figure 3

From: Salvianolic acid B inhibits RAW264.7 cell polarization towards the M1 phenotype by inhibiting NF-κB and Akt/mTOR pathway activation

Figure 3

Sal B inhibits RAW264.7 macrophage M1 polarization. (A) Form changes after RAW264.7 macrophages were treated with LPS (100 ng/mL) + IFN-γ (2.5 ng/mL) for 24 h. RAW264.7 macrophages were treated with Sal B (100, 150 or 200 μM) for 1 h and then stimulated with LPS (100 ng/mL) + IFN-γ (2.5 ng/mL) for 24 h. (B,C) iNOS (B) and Arg-1 (C) mRNA expression was measured by RT‒qPCR. D-F TNF-α (D), IL-6 (E) and IL-10 (F) secretion expression was evaluated by ELISA. The values are presented as the means ± SEMs. N = 3, p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***).

Back to article page