Figure 1
From: Atypical behavioral and thermoregulatory circadian rhythms in mice lacking a microbiome
Circadian manipulations and behavioral data collection. Data collection were conducted in two experimental cohorts. In Cohort 1, the order of circadian manipulations was as follows: Circadian entrainment to 12L:12D, Photic phase resetting (jet lag), Photic phase shift [acute light pulses at projected ZT15 (‘pZT15’), then at pZT22], Endogenous rhythm in constant darkness (DD), and Non-photic food entrainment (restricted feeding; RF). In Cohort 2, the order of circadian manipulations was as follows: Circadian entrainment to 12L:12D, Endogenous rhythm in DD, Photic phase resetting (jet lag), and Non-photic food entrainment (RF). Data sets from replication experiments were combined only if no statistically significant differences were observed between cohorts, which occurred in all instances except in the case of the photic phase resetting (jet lag) test (F1,319 = 69.318, P < 0.0001), therefore these data were reported separately for each cohort (see Fig. S5). Created with BioRender.com.
