Figure 7 | Scientific Reports

Figure 7

From: Pinocembrin suppresses oxidized low-density lipoprotein-triggered NLRP3 inflammasome/GSDMD-mediated endothelial cell pyroptosis through an Nrf2-dependent signaling pathway

Figure 7

Role of Pin in atherosclerotic lesions and CD31-TUNEL-positive cells in Apoe–/– mice. The atherosclerotic lesion model of Apoe−/− mice fed a high-fat-diet was established and then intragastrically administered with the vehicle (model group) and pinocembrin (Pin group, 20 mg/kg), once daily for continuously 14 weeks. In addition, a normal chow diet was fed to male C57BL/6 mice as the background control of Apoe−/− mice. (a) HE-stained aortic root cross-sections to examine the atherosclerotic lesion formation. Scale bar = 200 µm. (b) Lipid content stained by oil O red and bright green. Scale bar = 200 µm. (c) Collagen content stained by Masson. Scale bar = 200 µm. (d) Representative fluorescence micrographs depicting the CD31 and TUNEL staining images for endothelial cell with DNA damage in the aortic roots. Red: CD31; Green: TUNEL; Blue: nuclei. Scale bar = 20 µm. Data are described with mean ± SD of at least 3 different experiments. *P < 0.05; **P < 0.01 vs. model group.

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