Figure 5
From: Role of Methoprene-tolerant in the regulation of oogenesis in Dipetalogaster maxima
Effect of DmaxMet gene silencing on ovarian development. (a) Representative ovarian tissue showing terminal follicles from insects treated with either dsARG (control) or dsMet, two days after blood meal. (b) Terminal follicle size from dsRNA-treated insects. The results are shown as mean ± SD of three independent experiments and included samples from insects processed individually (n = 4). The statistical analyses were performed using Student’s t-test (***P < 0.001). (c) Effect of dsMet and dsARG treatment on Vg and Lp uptake by developing oocytes. Positive signal for Vg (red) and Lp (green) observed in the perioocytic space and oocyte membrane (arrows) as well as in the oocytes (asterisks) of dsARG-treated females (controls) was decreased or faintly detected in dsMet-silenced insects. Bars: 100 µm. FE (follicular epithelium); TF (terminal follicle); Tp (tropharium). Similar results were obtained in three independent experiments.
