Table 3 Studies of interest that analysed alizarin red S stains other than on otoliths.
From: New insights into detecting alizarin from autofluorescence in marked glass eels
Reference | Species | Material | Sample used | ARS light source | ARS excitation filter | ARS barrier or emission filter |
|---|---|---|---|---|---|---|
Present study | European eel Anguilla anguilla | Fluorescence reflectance imaging (Lumina LT) | Living whole-body | In the Blue In the Green | 450–480 nm 485–515 nm | 515–575 nm |
Epifluorescence binocular (SMZ25, Nikon) with a Nikon B-2A fluorescence filter | Fin rays | Blue light | 450–490 nm | 510 nm 515 nm | ||
Fluorescence spectroscopy with an Aqualog spectrofluorometer (Jobin Yvon) | Cellular content of fins | From UV to IR | 385–560 nm | 480–670 nm | ||
Liu et al. 2009 | Japanese flounder Paralichthys olivaceus | Epifluorescence microscope (OLYMPUS BX51) with an Olympus DP70 high resolution digital camera | Otoliths, fin rays and scales | UV Blue light Green light | 365 nm 490 nm 545 nm | 420 nm 515 nm 590 nm |
Bensimon-Brito et al. 2016 | Zebrafish Danio rerio | Stereo microscope (Leica MZ36) with a F-View II camera | Skeletal tissue imaging | Blue light Green light | 450––480 nm 510–550 nm | |
Caraguel et al. 2015 | European eel Anguilla anguilla | Epifluorescence microscope (OLYMPUS BX 51) | Otoliths | Green light | 530–550 nm | 590 nm |
Bashey 2004 | Guppies Poecilia reticulata | Epifluorescence microscope | Fin rays | Green light | 545 nm | 580 nm |
Crook and O’Mahony 2009 | Golden perch Macquaria ambigua | Stereomicroscope (MZ16 F Leica) with digital camera | Head, anal fin, caudal fin and otoliths | Green light | 560–600 nm | 610 nm |
Ibanez et al. 2013 | Nile Tilapia Oreochromis niloticus | Epifluorescence microscope (Zeiss Axio Start) | Otoliths, scales and caudal fin rays | Green light | 560–600 nm | 630–705 nm |
