Figure 5
From: Dicationic styryl dyes for colorimetric and fluorescent detection of nucleic acids
Applications of cationic styryl dyes for DNA detection in vitro (a–e) and for cellular nucleic acid detection (f). Sensitivity (a,b) and specificity (c,d) test of E. coli (a,c) and S. aureus (b,d) detection based on LAMP-4QL2+ assay, and gel electrophoresis results from the E. coli sensitivity assay (e). Lanes in (a), (e) 1: negative control, 2: 3.39 × 10−1, 3: 3.39 × 100, 4: 3.39 × 101, 5: 3.39 × 102, 6: 3.39 × 103 copies of DNA/reaction; (b) 1: negative control, 2: 3.3 × 10−2, 3: 3.3 × 10−1, 4: 3.3 × 100, 5: 3.3 × 101, 6: 3.3 × 102 copies of DNA/reaction; (c) 1: E. coli ATCC 25922, 2: E. coli MCCU0349, 3: E. cloacae, 4: S. aureus ATCC 25923, 5: S. epidermidis ATCC 12228, d) 1: S. aureus ATCC 25923, 2: S. aureus MCCU0357, 3: S. aureus MCCU0370 sample; 4: S. epidermidis ATCC 12228, 5: S. saprophyticus ATCC 15305, 6: E. coli ATCC 25922. (f) Fluorescence images of HeLa cells stained with BT2+(NEt2) and SYTO RNA select® Green (as individual dyes or co-stained). The cells were co-stained with DAPI in all cases (blue channel). Scale bars = 10 µm.
