Table 3 Comparison of the limit of detection of cationic styryl dyes for the determination of dsDNA by fluorescence and UV–visible spectrophotometry.

From: Dicationic styryl dyes for colorimetric and fluorescent detection of nucleic acids

Dyes

Detection method

Fluorescence

Absorption

λex (nm)/λem (nm)

LOD (ng/mL)

Af (nm)/Ai (nm)

LOD (ng/mL)

PY+

PY2+(C4)

480/600

480/600

276 (15 nM)

35 (1.9 nM)

520/480

520/480

5918 (321 nM)

583 (32 nM)

BT+

BT2+(NEt2)

565/600

565/600

4.1 (0.22 nM)

1.2 (0.07 nM)

560/530

560/530

3213 (174 nM)

973 (53 nM)

4QL+

4QL2+

548/670

548/670

57 (3.1 nM)

50 (2.7 nM)

600/550

600/550

1666 (90 nM)

433 (23.5 nM)

  1. Conditions: [Dye] = 2 μM, [DNA (in bp)] = 0.3–3 μM; all measurements were performed in 10 mM sodium phosphate buffer pH 7.0.
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