Figure 4 | Scientific Reports

Figure 4

From: Regulation of MMP9 transcription by ETS1 in immortalized salivary gland epithelial cells of patients with salivary hypofunction and primary Sjögren’s syndrome

Figure 4

Characterization of epithelial and progenitor cell markers in iSGECs by immunofluorescence. iSGEC-pSS1 (left) and iSGEC-nSS2 (right) were cultured on gelatin coated 8-well tissue culture slides for 48–72 h. Phosphorylated ETS1 (Thr38) (P-ETS1) displayed greater overall nuclear localization and intensity in iSGEC-nSS2 and was co-expressed with increased MMP9 levels in iSGEC-pSS1. ETS1 and LEF1 demonstrated relatively low expression levels in iSGECs when assessed by IF. iSGEC-nSS2 cells expressed Phos(T38)-ETS1 uniformly as small nuclear bodies interspersed with high- expressing whole-cell localized Phos(T38)-ETS1, regardless of MMP9 levels. The overall intensity and localization of ETS1 and LEF1 was unremarkable in either cell line or highlighted obvious differences in some cells. Representative regions (yellow) are highlighted and displayed adjacent their respective image. Images were captured at 20 × magnification and white scale bars represent 100 μm.

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