Figure 2
From: Localized, time-dependent responses of rat cranial bone to repeated mild traumatic brain injuries
Modest increases in bone volume and thickness of the interparietal bone two weeks after rmTBI. The interparietal bone region-of-interest (ROI) is depicted in (a), which consisted of 150 microCT images sampled at the midline, caudal to the Lambda suture margin (scale bar = 2 mm). Quantification of mineralized bone volume (b) and mineralized bone thickness (e) revealed an increase in 2× and 3× mTBI mice compared to sham controls (post-hoc, *p < 0.05, **p < 0.005); whereas no significant differences were observed between groups in total object volume (c) or surface area (d). Representative Sham and 2× mTBI greyscale microCT images (f) illustrate modest bone thickening with rmTBI (scale bar = 500 µm). In (g), bone marrow cavities (dark grey) were isolated from trabecular bone (light grey) in the diploë region of the interparietal bone scan ROIs for volumetric analysis (h), revealing a reduction in marrow cavity volume (one-way ANOVA p = 0.02; post-hoc n.s.). Representative superior-view, binary projections of the diploë (i) illustrate a subtle reduction in marrow cavities with injury (scale bar = 500 µm; black indicates marrow cavity and white indicates bone). N = 5–10/group; one-way ANOVAs with Tukey’s post-hoc as appropriate.
