Table 4 The binding energy of AFM1 with DNA molecules and interactions with nucleotides.
DNA molecule | DNA sequence | Free energy of binding (kcal/mol) | Inhibition constant (Ki) | Interacting nucleic acids (Chain:nucleotid) |
|---|---|---|---|---|
B-DNA dodecamer (1BNA) | 5′-CGCGAATTCGCG-3′ | − 8.08 | 1.20 µM | A:T8, B:A17 B:A18, B:T19 |
B-DNA dodecamer D (195D) | 5′-CGCGTTAACGCG-3′ | − 7.54 | 2.97 µM | A:A7, A:C9 B:A19, B:A20 |
DNA (1CP8) | 5′-TTGGCCAA-3′ | − 7.18 | 5.44 µM | A:G3, A:G4 A:C5, A:C6 B:C6 |
