Figure 3 | Scientific Reports

Figure 3

From: Teg58, a small regulatory RNA, is involved in regulating arginine biosynthesis and biofilm formation in Staphylococcus aureus

Figure 3

teg58 mediated biofilm formation is independent of PIA production and regulation of the target genes. (A) Static biofilm assay with isogenic strains in SH1000 background in TSB plus 0.25% glucose for 24 h. The sarA and agr mutant strains were used as controls. Δteg58, 220 bp chromosomal deletion strain; Δteg58(teg58), teg58 mutant complemented with pSK236 containing 450-bp teg58 with its native promoter. Means and standard deviations from triplicate experiments are shown. Student t-test (*P < 0.05, *** or ****P < 0.001). (B,D) Real-time qRT-PCR analysis for various genes as indicated along with the rpoB control were evaluated by qRT-PCR (2−ΔΔCT) using total RNA isolated from the respective strains at 24 h grown biofilm cells in TSB. The data were normalized against rpoB as the reference transcript for qRT-PCR. Relative expression of the genes was plotted against the wild type set as 1.0. “n.s.” indicate “not significant”. Pairwise comparation with Student t-test (*P < 0.05, **P < 0.005). (C) Dot bot showing PIA production in various strains. PIA production was detected with an anti-S. aureus PIA antibody, showing no changed in PIA production due to the teg58 deletion compared to the wild type and complement strains. Original blot is presented in Supplementary Material files Fig. X2.

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