Figure 5

Inhibition of biofilm formation by l-arginine. (A) Static biofilm formation for various strains in complete CDM containing 1% glucose for 24 h in a microtiter plate (Costar, USA). (B) Biofilm formation for various strains for 24 h in complete CDM containing 1% glucose and various concentrations of exogenous l-arginine in a microtiter plate format. (C) Biofilm formation for various strains as indicated in TSB supplemented with 0.25% glucose for 24 h in a microtiter plate under induced (+ ATc) (500 ng/ml) and uninduced (-ATc) conditions. V represents pALC2073 as the vector control; V + argGH, represents pALC2073 with argGH including ribosome binding site cloned under tetO-inducible promoter; ATc stands anhydrotetracycline which is inducer for the pALC2073 system. (D) Transcript analysis of 10 μg of total cellular RNAs isolated from cultures in (C) under various conditions and probed with argG probe. “+” and “−” indicate the inducible and uninducible conditions. The relative band intensity of various lanes was determined by Image J software (NIH) and the statistically significant was calculated considering the wild type as 100. Original blot is presented in Supplementary Material files Fig. X4. Means and standard deviations from triplicate experiments are shown. Student t-test (*P < 0.05, **P < 0.01; *** or ****P < 0.001) and “n.s” indicate “not significant”.