Figure 1 | Scientific Reports

Figure 1

From: Lack of adducin impairs the stability of endothelial adherens and tight junctions and may be required for cAMP-Rac1-mediated endothelial barrier stabilization

Figure 1

Functional analysis of endothelial barrier and distribution pattern of endothelial AJ and TJ. (A) TER measurements of confluent WT and Add-KO cell monolayers under basal conditions (N = 3 per group). Representative immunostainings and corresponding quantification analysis depicting the continuity of the fluorescence intensity at the junctions for (B) AJ proteins VE-Cadherin, β-catenin and plakoglobin or (C) TJ proteins claudin-5 and ZO-1 from WT and Add-KO cells (N = 3–4 per group). Arrows indicate areas of fragmented membrane staining; arrowheads indicate thin membrane signal. Data are represented as mean ± SEM; ****p < 0.0001.

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