Figure 2 | Scientific Reports

Figure 2

From: Lack of adducin impairs the stability of endothelial adherens and tight junctions and may be required for cAMP-Rac1-mediated endothelial barrier stabilization

Figure 2

Protein and mRNA expression analysis of junctional and junction-associated molecules. (A) Representative RT-PCR for AJ and TJ components from confluent WT and Add-KO monolayers under basal conditions. The graph shows the pixel intensity ratio for each and the corresponding loading control, B2M (N = 8 per group). (B) Representative Western blot of junctional and junctional-associated molecules. The graph denotes the ratio between the pixel intensity for each protein and α-tubulin normalized to the respective WT control (N = 5 per group). (C) Representative Western blot for the claudin-5 transcriptional repressor FoxO1. The graph presents the relative pixel intensity normalized to the WT controls (N = 4). Data are represented as mean ± SEM; *p < 0.05; **p < 0.01; ****p < 0.0001.

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