Figure 6 | Scientific Reports

Figure 6

From: Lack of adducin impairs the stability of endothelial adherens and tight junctions and may be required for cAMP-Rac1-mediated endothelial barrier stabilization

Figure 6

Protein expression, activation state of Rac1 and RhoA small GTPases and GTPase activity effects on TER. (A) Representative Western blot images for Rac1 and RhoA under basal conditions and respective pixel intensity quantifications presented as bar graphs. The data are normalized to the corresponding WT control (N = 4 per group). G-LISA small GTPases activation analysis of (B) Rac1 and (C) RhoA from confluent WT and Add-KO cells treated with Vehicle or F/R for 1 h. Activation status of (D) Rac1 and (E) RhoA from cells stimulated with Vehicle or CN04 for 2 h. Bar graphs show raw optical density (O.D.) measured with a wavelength of 490 nm (N = 4–7 per group). (F) TER measurements from confluent WT and Add-KO cell monolayers. Red-dashed line indicates the time of treatments. Data are normalized to the initial point of each corresponding group. “#” denotes statistically significant difference between Vehicle and CN04 treated groups (N = 3 per group). Data are represented as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001, # p < 0.0001.

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