Figure 2 | Scientific Reports

Figure 2

From: Human serum albumin (HSA) regulates the expression of histone-like nucleoid structure protein (H-NS) in Acinetobacter baumannii

Figure 2

Genetic analysis of genes of A. baumannii AB5075 or AB5075 pMBLe-hns cultured in LB broth or LB broth supplemented with 3.5% HSA. qRT-PCR of genes associated with quorum sensing, acdA, kar, and fadD, with quorum quenching, aidA, with biofilm, csuAB, with β-oxidation, fadA, with stress, katE and ywrO and metabolism phaC expressed in LB or LB supplemented with HSA. Fold changes were calculated using double ΔCt analysis. At least three independent samples were used, and four technical replicates were performed from each sample. The A. baumannii AB5075 cultured in LB was used as reference. Data are presented as mean ± SD. Statistical significance (P < 0.05) was determined by ANOVA followed by Tukey’s multiple-comparison test, one asterisks: P < 0.05; two asterisks: P < 0.01 and three asterisks: P < 0.001. This figure was performed using GraphPad Prism version number 9 (GraphPad software, San Diego, CA, USA, https://www.graphpad.com/).

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