Figure 3

Phenotypic analysis of biofilm formation. Biofilm assays performed with the AB5075 strain grown in LB or LB supplemented with 3.5% HSA, Δhns and Δhns pMBLe-hns strains cultured in LB, and AB5075 pMBLe-hns culture in LB or LB supplemented with 3.5% HSA. (A) Quantification of the radio of biofilm to total biomass in polystyrene wells. The mean ± SD is informed of three independent experiments. Statistical significance (P < 0.05) was determined by ANOVA followed by Tukey’s multiple comparison test, one asterisks: P < 0.05; two asterisks: P < 0.01 and three asterisks: P < 0.001. (B) Tubes of biofilm experiment after the stained with 1% crystal violet y removed the excess. A representative experiment is shown. This figure was performed using GraphPad Prism version number 9 (GraphPad software, San Diego, CA, USA, https://www.graphpad.com/).