Figure 1
From: Passage number affects differentiation of sensory neurons from human induced pluripotent stem cells
Morphology and pluripotency of the three iPSC lines (06401-2sb, CMC226, rsAA-12) at different passage numbers (low, intermediate, and high). (A) Phase-contrast images of three iPSC cell lines at different passage numbers. Scale bar = 1000 microns. (B) The average proportion of pluripotent cells in iPSCs at different passage numbers based on measurements of the expression of Sox2, Oct3/4, and Nanog using flow cytometry. (C) The expression level of SOX2, OCT3/4, and NANOG in iPSCs at different passage numbers based on RNA-seq. LP = low-passage, IP = intermediate-passage, HP = high-passage.
