Figure 2

The morphology and expression of neuronal markers peripherin and βIII-tubulin in iPSC-dSNs differentiated from iPSCs at each of low, intermediate, and high passage numbers. (A) Representative immunofluorescent images of neurons stained for neuronal markers peripherin and βIII-tubulin. Scale bar = 200 microns. (B) Average expression levels of TUBB3 in iPSC-dSNs derived from iPSCs with different passage numbers by RNA-seq. (C) Average expression levels of PRPH in iPSC-dSNs derived from iPSCs with different passage numbers by RNA-seq. (D) Representative phase-contrast images of iPSC-dSNs derived from iPSCs with different passage numbers. Scale bar = 200 microns. LP = low-passage, IP = intermediate-passage, HP = high-passage; *FDR = 0.005.