Table 1 Source information for the materials used in neuronal differentiation and immunofluorescence.
From: Passage number affects differentiation of sensory neurons from human induced pluripotent stem cells
Product | Manufacturer | Catalog number |
|---|---|---|
Small molecules | ||
StemMACS™ LDN-193189 | Miltenyi Biotec | 130–103-925 |
StemMACS™ A83-01 | Miltenyi Biotec | 130–105-333 |
CHIR 99021 | BioVision Inc | 1677–25 |
RO4919097 | Selleck Chemical LLC | S157510MG |
SU 5407 | Tocris | 3300 |
Retinoic acid | Sigma Aldrich | R2625 |
Growth factors | ||
Human NT-3 Recombinant Protein | Gibco | PHC7036 |
Human beta-NGF Recombinant Protein | Gibco | PHG0126 |
Human GDNF Recombinant Protein | Gibco | PHC7045 |
Human BDNF Recombinant Protein | Gibco | PHC7074 |
Immunofluorescence antibodies | ||
Anti-Peripherin antibody | Abcam | ab4666 |
Anti-beta III Tubulin antibody | Abcam | ab78078 |
