Table 3 Apparent coenzyme dissociation constants for purified recombinant DepBRleg and coenzyme variants.

From: Structure–function characterization of an aldo–keto reductase involved in detoxification of the mycotoxin, deoxynivalenol

Enzyme

Kd (µM)

KdNADPH/KdNADH

NADPH

NADH

DepBRleg

11.4 ± 0.84

67.3 ± 2.5

0.17

R290E

123 ± 6.2

119 ± 9.8

1.03

R290N

59.5 ± 4.2

77.7 ± 3.6

0.76

Q294E

97.9 ± 13

91.2 ± 10

1.07

K217E

68.6 ± 8.7

87.1 ± 3.4

0.79

K217M

74.1 ± 4.6

82.9 ± 3.0

0.89

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