Figure 2
From: Sequence-dependence of Cy3 and Cy5 dyes in 3ʹ terminally-labeled single-stranded DNA
Variations in Cy3 and Cy5 fluorescence intensity as a function of sequence identity in 3ʹ-labeled single-stranded DNA oligonucleotides. The relative fluorescence intensity of Cy3 and Cy5-labeled 5mers was ranked from most to least intense (highest recorded fluorescence and its corresponding 5mer = 1). For Cy3 fluorescence the intensity falls by ≈ 65% and Cy5 by ≈ 73%. A list of the brightest and darkest fluorescent 5mers is shown to the top and bottom part of the panel, respectively.
