Figure 4
From: Sequence-dependence of Cy3 and Cy5 dyes in 3ʹ terminally-labeled single-stranded DNA
Influence on fluorescence intensity, expressed as deviation from mean fluorescence, of each X3X4X5 trinucleotide combination over each possible X1X2 dinucleotide in 3ʹ Cy3-labeled X1X2X3X4X5 pentamers. Mean values are corrected for background fluorescence then standardized across the entire range of Cy3 fluorescence signals. Large deviations are seen for all possible 3ʹ-XGCGC and 3ʹ-GCGXX permutations.
