Table 2 Summary of the most relevant findings and observations in the sequence-dependence of 3ʹ-Cy3 and 3-Cy5 labeled single-stranded DNA.
From: Sequence-dependence of Cy3 and Cy5 dyes in 3ʹ terminally-labeled single-stranded DNA
Observations | 3ʹ-Cy3 | 3ʹ-Cy5 |
|---|---|---|
Fluorescent range (norm.) | 1–0.35 (65% variation) | 1–0.25 (75% variation) |
Most fluorescent sequence (#1) | 3ʹ-GCGCG | 3ʹ-GCGCG |
3ʹ-GCGCX score | Top 4 most fluorescent | Top 4 most fluorescent |
3ʹ-XGCGC score | #5, 6, 8, 12 | #5, 6, 11, 14 |
20-most fluorescent sequences | G-rich | G-rich |
20-least fluorescent sequences | C-rich | C-rich |
3ʹ-CCCCX score | #1009, 1013, 1023, 1024 | #1007, 1011, 1021 |
Least fluorescent sequence (#1024) | 3ʹ-CCCCG | 3ʹ-CCTTC |
