Figure 5

Effects of GSE and CE treatments on spinal IBA1 immunoreactivity in SCI mice at the end of the experimental period. (A) Representative histological images of the spinal cord immunostained against IBA1 of each group (scale bar 100 μm). (B) Histogram representing the percentage of reactive and nonreactive microglial cells in the spinal dorsal horn. (C) Examples of nonreactive and reactive microglial cells. Note that reactive cells have an amoeboid form, a larger nucleus and shorter branching processes than nonreactive cells. Images were captured at the same regions where astrogliosis was analyzed Data are expressed as the mean ± SEM. a–d: Groups not sharing a letter showed significant differences in %reactive cells, p < 0.05. Intragroup significant differences: *** p < 0.001%nonreactive vs %reactive. Experimental groups: Sham (n = 6; slices = 53), SCI + Saline (n = 5; slices = 34), SCI + GSE10 (n = 6; slices = 65), SCI + GSE15 (n = 4; slices = 47), SCI + GSE20 (n = 6; slices = 78), SCI + CE10 (n = 5; slices = 67), SCI + CE15 (n = 4; slices = 57).