Figure 2 | Scientific Reports

Figure 2

From: Activation of recombinational repair in Ewing sarcoma cells carrying EWS-FLI1 fusion gene by chromosome translocation

Figure 2

RAD51 foci formed in in SK-N-MC and WE-68 Ewing sarcoma cells. Exponentially growing cells were fixed with methanol and then reacted with the primary antibodies against RAD51 or CEMP-F, followed by the Alexa Fluor-labeled secondary antibodies. Nuclei were counterstained with DAPI. In each cell line, images were captured from at least ten visual fields using fluorescence microscope, and representative results are shown (A). RAD51 foci-positive cells were counted in ten visual fields (see also “Materials and methods” section). In three independent series of observations, the means and standard deviations were calculated. The results are compared in a bar chart (B).

Back to article page