Figure 1 | Scientific Reports

Figure 1

From: Novel chemical entities inhibiting Mycobacterium tuberculosis growth identified by phenotypic high-throughput screening

Figure 1

A high throughput screen for inhibitors of M. tuberculosis growth. (A) We tested a library of 98,347 compounds for activity. M. tuberculosis was grown in the presence of 20 µM test compound for 5 days in 384-well plates in duplicate. Growth inhibition was calculated relative to the control (DMSO). R2 using Pearson’s correlation coefficient is shown. Compounds with > 90% inhibition are boxed with a dashed-line. (B) Hits were reconfirmed in a dose response assay. 1070 hits were tested and the IC90 against M. tuberculosis in liquid culture determined. The number of compounds in each category of potency is indicated.

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