Figure 2 | Scientific Reports

Figure 2

From: Humanized liver TK-NOG mice with functional deletion of hepatic murine cytochrome P450s as a model for studying human drug metabolism

Figure 2

Generation of POR conditional knockout mice. (A) Representative PCR genotyping result for POR conditional knockout mouse. fl, floxed allele, wt, wild type, ntc, no template control. Full-length gel image was presented in Supplemental Fig. 6. (B) Western blot analysis of POR protein in liver microsomes from wildtype, POR cKO fl/fl, and POR cKO fl/null mice. Protein expression of mouse POR (arrow) assessed using immunoblotting with polyclonal antisera against POR. Gt, genotype, Pt, phenotype. Full-length blot image was presented in Supplemental Fig. 7. (C) Expression level of POR mRNA in the livers of wild-type mice (n = 6), POR cKO fl/fl (n = 9), and POR cKO fl/null mice (n = 6) were measured using qRT-PCR. The NADPH-cytochrome c reducing activity was determined to estimate POR activity in mouse liver microsomes. Data are presented as the mean ± SD. Statistically significant difference when compared with that in wild-type (TK-NOG) mice; ns, not significant, ****p < 0.0001. (D) Immunohistochemical staining of POR protein in livers from wild type, POR cKO fl/fl, and POR cKO fl/null mice. P, portal tract, C, central vein. Scale bar, 200 μm. Arrow-heads in POR cKO fl/fl panel indicate typical cells not express the POR protein.

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