Figure 2
Expression of ICOS in tumor and TDLN following RT. (a,b) Panc02-SIY tumors were implanted subcutaneously into flanks of C57BL/6 mice. Tumors were treated with 12 Gy radiation on day 15. Tumors and TDLNs were harvested from untreated (NT) or radiation treated (RT) animals for Nanostring analysis. (a) Within the tumor, NT and RT samples were compared to identify differentially expressed genes at (i) day 2 and (ii) day 7 post radiation. Volcano plots were generated using the − log10 p-value (y-axis) and log2 fold change in gene expression (x-axis). Genes were considered significant if the Benjamini–Yekutieli adjusted p-value was < 0.1 and the log2 fold change was >  ± 1.0. Significant genes, either up or down regulated, are represented as red circles. The ICOS gene is identified as a blue circle and genes that are not significant (not sig) are indicated as white circles. (b) The same analysis from (a) was used to identify significantly expressed genes between NT and RT samples collected from the TDLN at (i) day 2 and (ii) day 7 post treatment with radiation.
