Figure 5
Effect of ICOS antibody on the tumor environment following RT. (i) Experimental design. 2 × 105 CT26 cells were implanted subcutaneously into the flanks of Balb/c mice on day 0. On day 14 all mice in radiation groups were treated with 12 Gy radiation to the tumor using CT guidance. Mice were injected i.p. with 0.25 mg/kg ICOS antibody or in Control groups, mice were treated with equivalent dose of isotype control antibody on day 14 and tumors were harvested on day 21 for FACS analysis. (ii) Representative flow plots. (iii) Quantitative bar graphs of T cell populations of interest in the tumor 7 days following treatment. b. Quantitative bar graphs of myeloid populations of interest in the tumor 7 days following radiation treatment. Error bars SEM, ****p < 0.0001, ***p < 0.001, **p < 0.01, *, p < 0.05 as determined by unpaired student t test. N = 4 mice per group.
