Figure 1

The absence of PARP3 has no impact on cell proliferation and tumor growth in LN229 and T98G cells. (a) PARP3 is highly expressed in glioblastoma cell lines. The protein expression levels of PARP3 and actin were examined on nuclear protein extracts and by western blotting using the appropriate antibodies. Original blots are shown in the supplementary information file. (b) Western blot analysis of PARP3 and actin expression in the wild-type (WT) and two PARP3^−/−1, PARP3^−/−2 T98G clones selected upon screening and sequence analysis. Uncropped Western-blots are shown in the supplementary information file. (c) Graphs compare proliferation rates between the parental T98G (WT) and the PARP3^−/−1 and PARP3^−/−2 T98G cells. Experiments were performed three times. Mean values of triplicates ± s.d are indicated. (d) Upper panel, Western blot analysis of PARP3 and GAPDH expression in the wild-type (WT) and two PARP3^−/−3, PARP3^−/−18 LN229 clones selected upon screening and sequence analysis. Lower panel, Western blot analysis of PARP3 and actin expression in the wild-type (WT) and the PARP3^−/−3 LN229 cells with a stable expression of either the Flag control (Flag), Flag-PARP3^WT or Flag-PARP3^HE. Loading amounts that have been optimized for the figure are indicated. Uncropped Western-blots are shown in the supplementary information file. (e) Graphs compare proliferation rates between the parental LN229 (WT) and the PARP3^−/−3 LN229 cell lines expressing either the Flag control (Flag), Flag-PARP3^WT or Flag-PARP3^HE fusion proteins. Experiments were performed three times giving similar results. Mean values of triplicates ± s.d. of a representative experiment are indicated. (f) Relative tumor growth curves of xenografts derived from the wild type (WT) and the PARP3^−/−3 LN229 cell lines expressing either the Flag control (Flag), Flag-PARP3^WT or Flag-PARP3^HE fusion proteins. Mean RTV ± s.d. (n = 6 individual mice) are expressed compared to tumor volumes on day 7 for all cell lines.