Figure 3 | Scientific Reports

Figure 3

From: Adjunctive therapy with the Tie2 agonist Vasculotide reduces pulmonary permeability in Streptococcus pneumoniae infected and mechanically ventilated mice

Figure 3

Pulmonary and systemic immune response was not altered by VT therapy 30 h p.i. Vasculotide (VT) (500 ng/100 µl i.v.) or PBS, and ampicillin (0.4 mg/kg bw i.p.) or 0.9% saline were administered 22 h after Streptococcus pneumonia (S. pn.) infection (control: PBS + hyaluronidase). The 6-h ventilation phase began 24 h post infection (p.i). A second dose of VT together with 1 mg HSA was administered 1.5 h prior to termination of the experiment. Immune cells in the alveolar space were isolated from BALF, stained with antibodies binding leukocyte surface markers and differentiated by flow cytometry. Blood leukocytes were analyzed with a hemocytometer. Cytokines in BALF and plasma were measured by Multiplex Cytokine assay. (A) Neutrophils (PMN) per µl BALF, (B) Neutrophilis per µl blood, (C) IL-6 in BALF (pg/ml) and (D) IL-6 levels in plasma (pg/ml). Values are listed as median + IQR with minimum/maximum values, individual values are shown as dots (control n = 4, all other groups n = 7–8, Mann–Whitney-U-Test).

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