Table 6 Polymerase chain reaction primer sequences for real-time reverse transcription (RT-qPCR).

From: Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum)

Target genes

Primers (5′ → 3′)

Primer length (bp)

F-Igm-F

CAAGCCATCCGTTGACAAAGT

133

F-Igm-R

TATCCCCTTGCTCCATTCGT

F-Tcrβ-F

TGGAGCAGGTGTGAAGGTAG

138

F-Tcrβ-R

CCACCGAAGTAAGCCTCTCG

F-Csfr1-F

GGAGTTCTGAGCTGGTGGTC

135

F-Csfr1-R

AGGTGCGGACATTGCCATTG

F-MHC-I-F

GCGTTGGCAGTGACACATTC

151

F-MHC-I-R

AGTCCTGTTTACCCTCTGCT

F-MHC-2-F

ATTGCAGTCACGTACCTGTC

132

F-MHC-2-R

CGTCAATCACCAACCTGTGC

β-actin: F

AGGGAAATTGTGCGTGAC

114

β-actin: R

AGGCAGCTCGTAGCTCTT

  1. Where β-actin is cobia β-actin, IgM indicates Immunoglobulin, Csfr1 Colony-stimulating factor receptor 1, MHC-1 Major histocompatibility complex 1, MHC-2 Major histocompatibility complex 2, eflα elongation factor 1-alpha, Tcrβ T cell receptor beta.
Back to article page