Figure 4
From: Cell-free protein crystallization for nanocrystal structure determination

Comparison of crystal structure between PhC_CF20°C/24h and PhC_IC (PDB ID: 5gqm)28. (a) Superimposed structures of PhC_CF20°C/24h (green) and PhC_IC (magenta). (b,c) Close-up views of (b) CTP and (c) ATP/GTP binding sites in PhC_CF20°C/24h, (d,e) Close-up views of (d) CTP and (e) ATP/GTP binding sites in PhC_IC. The selected 2|Fo| − |Fc| electron-density maps at 1.0σ are shown in bule. Hydrogen bonds are indicated with yellow dotted lines.