Table 2 Crystallographic data of PhC_CF.
From: Cell-free protein crystallization for nanocrystal structure determination
20 °C/2 h | 20 °C/4 h | 20 °C/6 h | 20 °C/12 h | 20 °C/24 h | 4 °C/24 h | 10 °C/24 h | 15 °C/24 h | 25 °C/24 h | 20μL_ 20 °C/24 h | |
|---|---|---|---|---|---|---|---|---|---|---|
No. of loops | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
No. of indexed images | 1 | 91 | 1531 | 8754 | 12,846 | 0 | 258 | 3834 | 3807 | 12,355 |
Space group | I23 | I23 | I23 | I23 | I23 | I23 | ||||
Unit cell (Å) a = b = c | 104.3 | 103.3 | 104.4 | 104.4 | 103.7 | 103.6 | ||||
Resolution range (Å) | 50–2.50 (2.51–2.50) | 50–2.18 (2.19–2.18) | 50–1.80 (1.81–1.80) | 50–2.20 (2.21–2.20) | 50–1.87 (1.88–1.87) | 50–1.95 (1.96–1.95) | ||||
Observed reflections | 427,843 (6586) | 343,3242 (59,651) | 6,907,468 (114,912) | 1,489,024 (27,270) | 2,048,341 (33,158) | 6.106,562 (95,158) | ||||
Unique reflections | 6679 (148) | 9832 (253) | 17,297 (414) | 9786 (261) | 15,459 (367) | 13,641 (309) | ||||
Completeness (%) | 100 (100) | 100 (100) | 100 (100) | 100 (100) | 100 (100) | 100 (100) | ||||
Multiplicity | 64.1 (44.5) | 349 (236) | 399 (277) | 152.2 (105) | 133 (90) | 447.7 (308) | ||||
I/σ | 3.8 (1.24) | 7.3 (1.28) | 5.5 (1.53) | 4.8 (1.14) | 4.5 (1.33) | 7.3 (1.23) | ||||
CC1/2 | 0.9457 (0.5054) | 0.98821 (0.5854) | 0.9899 (0.5326) | 0.9759 (0.4000) | 0.9696 (0.4921) | 0.9908 (0.6707) |
