Table 1 Primer sequences used in the cDNA cloning, Quantitative real-time PCR and dsRNA synthesis.
Genes | Primer sequence (5′→3′) |  |  |
|---|---|---|---|
RACE | |||
Hsf1 | |||
F | TCACGGAAGTAGGGACGAATGT | Â | Â |
R | CACTGTGTTGTCTGGCATACTG | Â | Â |
5′ | AGAGACATTCGTCCCTACTTCCGT |  |  |
3′ | GTTACTGGTTCTCTACCTCTGGAT |  |  |
qRT-PCR | Â | R2 | E (%) |
Hsf1 | Â | 0.991 | 96.5 |
F | TCTGGTCCATCCCACACG | Â | Â |
R | CTGCCGCTGTTGTAGTGC | Â | Â |
Hsp90 | Â | 0.985 | 98.4 |
F | ACAACTTGGGAACAATCGCC | Â | Â |
R | ATAGAGATGTCAGCACCAGC | Â | Â |
Hsp70-1 | Â | 0.995 | 99.0 |
F | GAAGAACTTTGCTCTGACCT | Â | Â |
R | TATCCATTTTCGCATCAGCC | Â | Â |
Hsp70-3 | Â | 0.994 | 100.5 |
F | CCTACGGATTGGATAAGAACCTG | Â | Â |
R | GCAGTTGCTCGCACTTCAAATAG | Â | Â |
Hsp20 | Â | 0.990 | 106.1 |
F | ACCAACCCACTCTCCGCAC | Â | Â |
R | ACTCCACTCTGCTGGGCTG | Â | Â |
Hsp19.5 | Â | 0.988 | 99.8 |
F | TTCACCCGCCGTTACCTTCTGC | Â | Â |
R | TGCCTTTGGTGGAGCCTGGATG | Â | Â |
EF-1α |  | 1 | 95.3 |
F | TAGCCTTGTGCCAATTTCCG | Â | Â |
R | CCTTCAGCATTACCGTCC | Â | Â |
RPL29 | Â | 1 | 96.6 |
F | TCGGAAAATTACCGTGAG | Â | Â |
R | GAACTTGTGATCTACTCCTCTCGTG | Â | Â |
dsRNA synthesis | |||
dsBthsf1 -F | TAATACGACTCACTATAGGG | Â | Â |
CACATCAAAACTTAGAAATAATT | Â | Â | |
dsBthsf1 -R | TAATACGACTCACTATAGGG | Â | Â |
AGTCTTCTTCCATCTTTTGACTT | Â | Â | |
dsGFP -F | TAATACGACTCACTATAGGG | Â | Â |
CCTCGTGACCACCCTGACCTAC | Â | Â | |
dsGFP -R | TAATACGACTCACTATAGGG | Â | Â |
CACCTTGATGCCGTTCTTCTGC | Â | Â | |
