Figure 5
From: The cost of the circadian desynchrony on the Leydig cell function
CD changed mitochondrial function. Leydig cells from both groups were individually isolated at different time points during 24 h and followed by measurements to estimate mitochondrial mass and function: mitochondrial mass was estimated through mitotrack assay (a) and Mt-Nd1/B2m ratio (d), mitochondrial membrane potential (ΔΨ) was estimated through TMRE measurement (b) and mitochondrial energetic function was estimate through ATP measurement (c). CD decreased mesor of ΔΨ and ATP production. Mitochondrial biogenesis were estimated by transcriptional activity for several genes (e–j); mitochondrial fusion (k–m); mitochondrial fission (n,o) and mitophagy (p–r). Statistical significance: *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001 determined by two-way ANOVA.
