Figure 1
From: Cocaine regulation of Nr4a1 chromatin bivalency and mRNA in male and female mice

Methods used to investigate regional transcriptional and epigenomic profiles following cocaine exposure. (A) Timeline for investigator-administered cocaine (20Â mg/kg; i.p) paradigm. (B) Coronal brain slice illustrations showing the brain regions taken for single sample sequencing (Top to bottom: prefrontal cortex (PFC), striatum (STR), hippocampus (HPC)). (C) Schematic showing the sequential ChIP protocol that includes two immunoprecipitations.