Figure 4
From: Cocaine regulation of Nr4a1 chromatin bivalency and mRNA in male and female mice
Cocaine alters the transcriptomic profile of Nr4a1 in the STR of females and the transcriptomic and epigenetic profile of Nr4a1 in the PFC of females. (A) There was an increase in mRNA levels of Nr4a1 (as examined by primer pairs that cover the junction between exons 4/5 and exons 6/7) in the female mouse STR following 10 days of investigator-administered cocaine (n = 4–6/group). There was no change in (B) H3K4me3 (n = 6/group), (C) H3K427me3 (n = 5–6/group), (D) H3K4me3/H3K27me3 bivalency (n = 6/group), or (E) H3K27me3/H3K4me3 bivalency (n = 5–6/group) at Nr4a1 in the female mouse STR after 10 days of investigator-administered cocaine. (F) There was no change in mRNA levels of Nr4a1 as measured by independent primer pairs that cover the junction between exons 4/5, and a trending increase in mRNA levels of Nr4a1 as measured by independent primer pairs that cover the junction between exons 6/7 in the female mouse HPC following 10 days of investigator-administered cocaine (n = 7–8/group). There was no change in (G) H3K4me3 (n = 7–8/group), (H) H3K27me3 (n = 6–8/group), (I) H3K4me3/H3K27me3 (n = 7–8/group), or (J) H3K27me3/H3K4me3 bivalency (n = 6–8/group) at Nr4a1 in the female mouse HPC following 10 days of investigator-administered cocaine. (K) There was an increase in mRNA levels of Nr4a1 (as measured by independent primer pairs that cover the junction between exons 6/7, but not exons 4/5) in the female mouse PFC following 10 days of investigator-administered cocaine (n = 7–8/group). There was no change in (L) H3K4me3 (n = 7/group), but there was an increase in (M) H3K27me3 (n = 7/group) at Nr4a1 following 10 days of investigator-administered cocaine in the female mouse PFC (n = 7/group). (N) There was no change in H3K4me3/H3K27me3 bivalency (n = 7/group) or (O) H3K27me3/H3K4me3 bivalency at Nr4a1 in the female mouse prefrontal cortex following 10 days of investigator-administered cocaine (n = 7/group). *p < 0.05. #p < 0.10. Data are shown as mean ± SEM. STR, striatum; HPC, hippocampus; PFC, prefrontal cortex.
