Figure 3 | Scientific Reports

Figure 3

From: Interphotoreceptor matrix proteoglycans IMPG1 and IMPG2 proteolyze in the SEA domain and reveal localization mutual dependency

Figure 3

Mutations in the SEA-2 domain of IMPG1 prevent proteolysis. (a,b) IMPG1 SEA-2 amino acid sequence and predicted tertiary structure, including mutations linked to retinitis pigmentosa pathology in humans (red letters). Protein structure prediction made with the AlphaFold server. (c) Western blot assay detecting IMPG1 molecules synthesized by HEK293 cells expressing either GFP as a negative control, IMPG1-WT, IMPG1-L583P, or IMPG1-L630F transgenes. (d) Protein expression quantification of proteolyzed (blue) and non-proteolyzed (green) IMPG1. Results were obtained in triplicate and referenced with total protein stain. Bars are mean  ±  SEM of three samples. Both WT groups are significantly different (*p  <  0.01) from each mutant by two-way T-test. All western blot samples were deglycosylated before loading onto the gel; untreated protein and total protein are in Fig. S2.

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