Figure 1
The bacterial loads and histopathology of tissues during infection with KYMR116. (A) The number of bacteria in the tissue was quantified at 6, 24, and 48 h after infection with KYM116. The results represent the mean CFU/wound (n = 5). (B) The density of the infiltrating inflammatory cells was quantified using three areas randomly chosen in the abscesses. The data indicate the means of the number of cells per 104 µm2 (n = 3). (C) Emerged fibroblasts were quantified using three areas randomly chosen in the abscesses. The data indicate the means of the number of cells per 4 × 104 µm2 (n = 3). (D) Mouse skin tissues observed by haematoxylin and eosin (H&E) staining at 48 h after infection. Data are representative of three mice in each group. Cells enclosed in dotted lines are fibroblasts. Scale bars, 1 mm in a and e, 200 µm in b and f, and 50 µm in c, d, g and h. (E) Collagen and elastin fibres in the mouse skin were detected by Elastica van Gieson staining. The micrographs of a-h in (D) and (E) are the areas indicated by squares a-h, respectively. An asterisk in (D)-b indicates the lesion of infection in the deep tissue. ‘KYMR116 + ϕ’ are the samples prepared from mice coinoculated with phiMR003 and KYMR116. Error bars indicate standard errors of the means (SEMs). *, P < 0.05; **, P < 0.01. Scale bars, 1 mm in a and e, 200 µm in b and f, 100 µm in c and g, and 50 µm in d and h.
