Figure 1 | Scientific Reports

Figure 1

From: Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare

Figure 1

Characterization of uterine extracellular vesicles (uEVs). (A) Representative images (transmission electron microscopy) of EVs samples isolated from uterine lavages from pregnant mares on days 10 (P10), 11 (P11), 12 (P12), and 13 (P13), and from cyclic mares (controls) on days 10 (C10) and 13 (C13) after final ultracentrifugation and referred as uEVs. (B) Western blot characterization of uEVs (100,000 g) and the 12,000 g pellet for known exosomal protein markers CD9, TSG101, ALIX (PDCD6IP) and HSP70. (C) Comparison of uEVs concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300). (D) Comparison of uEVs protein concentration across samples measured by Pierce™ BCA protein assay. (E) Comparison of uEVs RNA concentration across samples measured by Quantus™ Fluorometer.

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