Table 1 The results of DZN 20 mg/kg and CH 50 mg/kg, and DZN plus CH (12.5, 25, and 50 mg/kg) on splenocyte subtypes in BALB/c mice.

From: Potential protective effects of chrysin against immunotoxicity induced by diazinon

 

DZN

DZN + CH1

DZN + CH2

DZN + CH3

CH3

CTX

Control

Spleen Cell

Numbers (× 107)

8.38 ± 0.03*

8.61 ± 0.16

9.37 ± 0.25

9.61 ± 0.15 + 

10.43 ± 0.58

8.08 ± 0.04**

9.54 ± 0.11

CD19+

Cell (%)

32.20 ± 3.08

34.80 ± 1.31

35.4 ± 0.6

37.61 ± 0.81

39.60 ± 0.5

18.60 ± 1.32***

37.60 ± 3.18

CD19+

content

2.53 ± 0.26*

2.99 ± 0.12

3.31 ± 0.07

3.61 ± 0.11 +  + 

4.12 ± 0.23

1.50 ± 0.10***

3.59 ± 0.32

CD49b+

cell (%)

3.94 ± 0.53***

4.26 ± 0.55

4.87 ± 0.48

6.07 ± 0.4 + 

6.88 ± 0.23

3.90 ± 0.24***

7.72 ± 0.44

CD49b+

content

(× 107)

0.33 ± 0.04***

0.36 ± 0.04

0.45 ± 0.04

0.58 ± 0.01 +  + + 

0.71 ± 0.03

0.31 ± 0.2***

0.73 ± 0.04

CD3+

cell (%)

48.2 ± 1.59***

53.8 ± 1.95

58.4 ± 0.92 + + 

57.4 ± 2.73 + + 

72.6 ± 1.32

43.4 ± 1.2***

70.4 ± 1.69

CD3+

content

3.37 ± 0.34

5.12 ± 0.32

5.44 ± 0.17

5.09 ± 0.19

6.16 ± 0.32

5.80 ± 0.21***

5.62 ± 0.10

CD4+

cell (%)

40.20 ± 4.09*

45.4 ± 1.36

44 ± 1.51

45.6 ± 1.63

44.8 ± 1.24

39.8 ± 0.58*

49.20 ± 1.06

CD4+

content

3.37 ± 0.34**

3.91 ± 0.17

4.12 ± 0.18

4.38 ± 0.16 + 

4.67 ± 0.29

3.21 ± 0.06***

4.69 ± 0.14

CD8+

cell (%)

20.04 ± 0.79*

20.42 ± 0.47

21.01 ± 0.41

22.64 ± 0.26

25.04 ± 0.59

16.34 ± 1.11***

24.37 ± 1.87

CD8+

content

1.68 ± 0.06**

1.75 ± 0.03

1.97 ± 0.05

2.17 ± 0.05 + 

2.61 ± 0.18

1.32 ± 0.09***

2.32 ± 0.14

  1. Data showed as mean ± SD, (*) showed significant effect compared to control, ( +) comparison with the diazinon-treated group. p < 0.05 and (***) or (+ +  + +) p < 0.001. DZN caused a significant reduction in the percentage and number of TCD4+, TCD8+, and NK cells. However, in the presence of CH, it could somehow neutralise the effect of DZN and increase the percentage and the number of immune cells.
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