Table 4 The results of DZN 20 mg/kg, CH 50 mg/kg, and CH and DZN plus CH (12.5, 25, and 50 mg/kg) on cytokine and total IgM and IgG production in BALB/c mice.

From: Potential protective effects of chrysin against immunotoxicity induced by diazinon

Parameters

DZN

DZN + CH1

DZN + CH2

DZN + CH3

CH3

CTX

Control

IL-4

(pg/mL)

26.23 ± 0.34***

25.87 ± 0.82

24.91 ± 1.14

22.76 ± 0.51 +  + 

22.52 ± 0.27

11.63 ± 0.28***

21.67 ± 0.41

IL-10

(pg/mL)

32.47 ± 0.50***

30.66 ± 1.08

31.88 ± 0.32

29.41 ± 0.49 + 

24.44 ± 0.36

12.11 ± 0.72***

26.36 ± 0.56

IFN-γ

(pg/mL)

16.55 ± 0.36***

17.93 ± 0.45

18.46 ± 0.53

20.62 ± 0.76 +  + + 

22.98 ± 0.49

14.24 ± 0.50***

24.29 ± 0.66

TGF-β

(pg/mL)

34.67 ± 0.89***

31.02 ± 0.59 +  + 

29.33 ± 0.82 +  +  + 

28.18 ± 0.83 +  + + 

24.24 ± 0.22

14.52 ± 0.69

26.17 ± 0.68

IL-17

(pg/mL)

19.27 ± 0.15***

19.07 ± 0.13

18.80 ± 0.30

17.14 ± 0.21 +  + 

14.15 ± 0.26

11.72 ± 0.71***

13.94 ± 0.21

IgM

(μl/ml)

1022.4 ± 7.19***

1044 ± 15.03

1087 ± 2.7 +  + 

1103 ± 4.77 +  + + 

1154 ± 9.27

879 ± 7.48***

1106 ± 23.12

IgG

(μl/mL)

8236 ± 23.99***

8304 ± 32.96

8595 ± 13.23 +  +  + 

9009 ± 20.46 +  + + 

9823 ± 13.18 ***

7524 ± 20.91 ***

8541 ± 24.29

  1. Data showed as mean ± SD, (*) shows the significant changes compared to controls ( +) comparison with DZN-treated group. p < 0.05 and (***) or (+ +  + +) p < 0.001. Cytokines measurement in the DZN group showed that the production of IFN-γ in the DZN group was significantly down-regulated compared to the control group. However, the significant impact of CH on the neutralisation of toxicity effects of DZN on IFN-γ was dose-dependent and only taken at the highest concentration of CH 50 mg/kg.
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